2010 ALS User's Meeting Workshop

Infrared Spectromicroscopy:

The Berkeley Synchrotron Infrared Structural Biology (BSISB) Program


This workshop aims to familiarize potential users of the new ALS infrared  beamline with the wide variety of science that can be performed using the novel tools available at the BSISB facility.  BSISB is directed by Hoi-Ying Holman, and is funded by the Biology and Environmental Research office within the Department of Energy.  After several talks highlighting multiple scientific areas, Dr. Holman will lead a hands-on session that will demonstrate how to prepare living samples for SR-FTIR analysis and how to acquire high-quality data.

Organizers:  Hans Bechtel, Hoi-Ying Holman, Michael Martin and Peter Nico


Thursday, October 14, 2010

Room Location: 50 Auditorium (Part of the main ALS Users' Meeting)
08:00 Continental Breakfast
08:30 - 09:00 Hoi-Ying Holman Invited Award Talk: "Looking at the Deep-Sea Oil Plume in the Gulf of Mexico: Enrichment of Oil-Degrading Bacteria via Mid-Infrared Spectroscopy Microanalysis"
09:30 - 10:00 Break


Room Location: 15-300
10:00 - 10:10 Hoi-Ying Holman Welcome
10:15 - 10:40 Peter Nico / Michael Martin Beamline 5.4 and the ALS IR program
10:45 - 11:15 John Vogel, USDA Brachypodium distachyon: a new model for grass cell walls
11:20 - 11:50 Kathy Gough, U. Manitoba High Definition Imaging of fungi and brain tissue with IRENI – Discoveries enabled by the new technology
12:00 - 13:15 Lunch
13:20 - 13:50 Eleanor Blakeley, LBNL Mapping and characterization of low-dose radiation effects
13:55 - 14:25 Christer Jansson, LBNL Ecology Dept. Exploring Cyanobacteria for Biofuel Synthesis and Carbon Sequestration
14:30 - 15:00 Francis Blankenberg, Stanford School of Medicine Oxidative Stress in Apoptosis and Mitochondrial Related Disease
15:05 - 15:30 Break
15:35 - 16:05 Jamie Cate, UC Berkeley Chemistry A tale of two fungi:  Improving biofuel production from plant biomass
16:10 - 16:45 Discussion
17:30 - 19:30 Awards Dinner ALS Patio

Friday, October 15, 2010

Spectroscopy Solutions and Introduction to FTIR and Raman Spectroscopies

Joint with Thermo-Fisher Scientific
08:00 Continental Breakfast ALS Patio
Room Location: 15-300 then Beamline 5.4
09:00 - 09:30 Mark McMahon, Thermo-Fisher Introduction to FTIR, Raman and NIR Spectroscopy
09:35 - 10:05 Michael Martin How does FTIR work and FTIR Microscopy
10:05 - 10:30 Break
10:35 - 11:05 Alex Rzhevskii, Thermo-Fisher Intro to Raman Microscopy
11:10 - 11:50 Hans Bechtel Tour and orientation of the Beamlnie 5.4 BSISB facility
12:00 - 13:15 Lunch
13:15 - 14:45 All Hands on work with FTIR and Raman Microscopes
14:45 - 15:15 Hoi-Ying Holman
Working with live cells: Practical sample prep and handling
15:15 - 15:45 Break
15:45 - 16:30 Hoi-Ying Holman Microfluidics demonstration (Buiding 46)

Thank you for a great workshop!

Here are some photos taken during the hands on session at Beamline 5.4: